Efficient TGFβ-induced epithelial–mesenchymal transition depends on hyaluronan synthase HAS2

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Overproduction of hyaluronan by expression of the hyaluronan synthase Has2 enhances anchorage-independent growth and tumorigenicity.

Hyaluronan (HA) has long been implicated in malignant transformation and tumor progression. However, due to the lack of molecular tools to directly manipulate production of HA, which does not require a core protein for its synthesis, our understanding of the role of HA in tumor cells has been largely circumstantial. In this study, we genetically manipulated the production of HA by transfection ...

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Mechanisms of TGFβ-Induced Epithelial–Mesenchymal Transition

Transitory phenotypic changes such as the epithelial-mesenchymal transition (EMT) help embryonic cells to generate migratory descendants that populate new sites and establish the distinct tissues in the developing embryo. The mesenchymal descendants of diverse epithelia also participate in the wound healing response of adult tissues, and facilitate the progression of cancer. EMT can be induced ...

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HAS 2 ( hyaluronan synthase 2 )

Hyaluronan is catalyzed by three membraneembedded HA synthases, although HAS2 is the only essential gene. The isozymes are coded by 3 separate genes that, although related, are unlinked in the chromosome. Regulation occurs at trans-cription, posttranslational levels, as well as, by alternative splicing, differential sub-cellular locali-zation and epigenetic processes. Hyaluronan deposits in the...

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Generation of branched tubes from an epithelial bud is a fundamental process in development. We hypothesized that induction of hyaluronan synthase (Has) and production of hyaluronan (HA) drives tubulogenesis in response to morphogenetic cytokines. Treatment of J3B1A mammary cells with transforming growth factor-β1 or renal MDCK and mCCD-N21 cells with hepatocyte growth factor induced strong and...

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ژورنال

عنوان ژورنال: Oncogene

سال: 2012

ISSN: 0950-9232,1476-5594

DOI: 10.1038/onc.2012.475